Effectiveness Testing of Mahogany Seed Ethanol Extract (EEBM) as an Antidiarrheal Agent in Male Wistar Strain White Mice
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Abstract:
Background: Increased bowel motions and a looser stool consistency than usual are signs of diarrhea. Alkaloids, which are present in traditional plants like mahogany seeds and can reduce intestinal motility and excess fluid secretion, are one of the secondary metabolites utilized as an antidiarrheal.
Aims: The purpose of this study was to determine the effectiveness of mahogany seed ethanol extract as an antidiarrheal agent in male Wistar strain white mice.
Methods: Using 96% ethanol as a solvent, extraction was carried out by maceration. 50 mg/kgBW, 100 mg/kgBW, and 200 mg/kgBW were the dosages utilized; loperamide was employed as a comparator. Six groups of twenty-four experimental animals were given 0.5 mL of oleum ricini to induce diarrhea. The intestinal transit method (measuring the length of the norit passage through the intestine) and the defecation method (frequency, consistency, and weight of feces) were then used to make observations. One-way ANOVA was used to evaluate the data, and Bonferroni's post hoc test was then used.
Result: The extract was most successful in producing antidiarrheal effects at a dose of 200 mg/kgBW, according to the results of the oleum ricini-induced antidiarrheal activity test. The diarrhea group did not differ significantly from the 50 mg/kgBW and 100 mg/kgBW dose groups in the initial parameters of diarrhea, but they did differ significantly from the loperamide and 200 mg/kgBW dose groups.
Conclusion: Ethanol extract of mahogany seeds (EEMB) exhibited antidiarrheal effects at doses of 50 mg/kgBW, 100 mg/kgBW, and 200 mg/kgBW in experiments on male white rats. Therefore, EEMB may be a potential antidiarrheal drug for further pharmacological development.
Keywords: Antidiarrheal , Defecation method, Intestinal transit method, Mahogany seed extract Swietenia mahogani (L.) Jacq
Copyright (c) 2026 Naili Fadlir Rahma Zahiri, Miming Andika, Billy Harnaldo Putra
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References
Ambari, Y. (2018). Uji Aktivitas Antidiare Ekstrak Etanol Daun Salam ( Eugenia polyantha Wight ) Pada Mencit Putih ( Mus musculus) Jantan Galur Balb-C. Journal of Pharmaceutical Care Anwar Medika Artikel, 1(1), 25–33. https://doi.org/http://dx.doi.org/10.36932/jpcam.v1i1.6
Andika, M., Yesika, R., & Fitriani, O. S. (2022). The Effects of Bisoprolol on Reduction of Average Arterial Pressure and Heart Rate in Male White Rats Hypertensions and Hypertension Complications Of Hypercholesterolemia. JOPS (Journal Of Pharmacy and Science), 5(2), 11–20. https://doi.org/https://doi.org/10.36341/jops.v5i2.2445
Anggraeni, R., Alifiar, I., & Suhendy, H. (2020). Uji Aktivitas Daun Mareme (Glochidion Borneense (Müll Arg) Boerl) Sebagai Antidiare Pada Mencit Jantan Galur Swiss Webster. Jurnal Farmaku (Farmasi Muhammadiyah Kuningan), 5(2), 59–69. https://doi.org/10.55093/jurnalfarmaku.v5i2.139
Dairo, M. D., Ibrahim, T. F., & Salawu, A. T. (2017). Prevalence and determinants of diarrhoea among infants in selected primary health centres in Kaduna north local government area, Nigeria. The Pan African Medical Journal, 28, 109. https://doi.org/10.11604/pamj.2017.28.109.8152
Department of Health of The Republic of Indonesia. (1979). Indonesian pharmacopoeia, 3rd edition. Department of health of the republic of Indonesia, Jakarta.
Dharmayanti, I., & Tjandrarini, D. (2020). Peran Lingkungan Dan Individu Terhadap Masalah Diare Di Pulau Jawa Dan Bali. Jurnal Ekologi Kesehatan, 19, 84–93. https://doi.org/10.22435/jek.v19i2.3192
Fadhil, M., Desnita, E., Elianora, D., Farmasi, B., Baiturrahmah, F. K. G. U., Pedodonasia, B., Baiturrahmah, F. K. G. U., Raya, J., Pass, B., Sei, K. M., & Kunci, K. (2017). Uji Efektifitas Ekstrak Biji Mahoni (Swietenia Mahagoni (L.) Jacq) Sebagai Antipiretik Pada Tikus Wistar (Rattus Norvegicus). Jurnal B-Dent, 4(2), 141–149. https://doi.org/10.33854/JBDjbd.106
Fajar, I. R. F., & Cahyo, H. D. (2020). Uji Aktivitas Ekstrak Etanol Daun Sawo Manila ( Manilkara zapota L ) Sebagai Antidiare Terhadap Mencit Putih Jantan ( Mus musculus ). Iontech, 01(01), 17–25. https://doi.org/https://doi.org/10.62702/ion.v1i1.24
Iryanto, A. A., Joko, T., & Raharjo, M. (2021). Literature review: risk factors of diarrhea incidence in toddlers in indonesia. Journal of Environmental Health, 11(1), 1–7. https://doi.org/10.47718/jkl.v10i2.1166
Kartikasari, Suhaimi, S., & Dian. (2020). Uji Antidiare Granul dari Ekstrak Etanol Daun Kratom (Mytragina specioca Korth) terhadap Mencit Putih Jantan (Mus musculus L). Jurnal Ilmu Kefarmasian Indonesia, Vol 18 No 1 (2020): JIFI, 101–108. http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/787/529
Kolo, S. M. D., Siburian, R. A. F., Lulan, T. Y. K., Biologi, P. S., Pendidikan, F. I., & Timor, U. (2016). Produksi Biodiesel dari Minyak Biji Jarak Pagar ( Jatropha curcas L .). Jurnal Pendidikan Biologi, 1(1), 6–8. https://doi.org/https://www.neliti.com/publications/377262/produksi-biodiesel-dari-minyak-biji-jarak-pagar-jatropha-curcas-l
Manek, M. S., Klau, M. E., & Beama, C. A. (2020). Uji Aktivitas Antidiare Ekstrak Etanol Daun Sirih (Piper betle L.) Pada Tikus Putih Jantan Galur Wistar Yang Diinduksi Oleum Ricini Maria. CHMK Pharmaceutical Scientific Journal, 3(April), 147–151.
Maryam, F., Subehan, & Musthainah, L. (2020). Isolasi Dan Karakterisasi Senyawa Steroid Dari Ekstrak Biji Mahoni ( Swietenia mahagoni Jacq .). Jurnal Fitofarmaka Indonesia, 7(2), 6–11. https://doi.org/10.33096/jffi.v7i2.647
Ministry of Health of The Republic of Indonesia. (2017). Indonesian Herbal Pharmacopoeia, 2nd edition. Ministry Of Health Of The Republic Of Indonesia.
Muthia, R., Musfirah, Y., & Candrakirana, S. (2019). Test Antidiarrhael Activity of Ethanolic Extract Morinda citrifolia against Mus musculus Induced by Bacter. Jurnal Borneo Journal of Pharmascientech, 03(01). https://doi.org/https://doi.org/10.51817/bjp.v3i1.217
Putri, A. H., Busman, H., & Nurcahyani, N. (2016). Uji Efektivitas Ekstrak Rimpang Rumput Teki (Cyperus rotundus L .) Dengan Obat Imodium Terhadap Antidiare Pada Mencit (Mus musculus L.) Jantan Yang Diinduksi Oleum Ricini. Jurnal Biologi Eksperimen Dan Keanekaragaman Hayati, 3(2), 25–32. https://doi.org/https://doi.org/10.23960/jbekh.v3i2.81
Rahayuningsih, N., Assyifa, S. N. H., & Rahmiyanii, I. (2021). Uji Aktivitas Antidiare Infusa Kulit Buah Delima Putih ( Punica granatum L .) pada Mencit Jantan Swiss Webster dengan Metode Oleum Ricini. Jurnal Ilmu Kefarmasian Indonesia, 19(1), 62–67.
Rambe, R., Gultom, E., Ginting, O., & Diana, S. (2021). Uji Efektivitas Antidiare Ekstrak Etanol Daun Mengkudu (Morinda citrifolia L.) Terhadap Mencit Jantan Dengan Metode Transit Intestinal. Forte Journal, 1, 1–11. https://doi.org/10.51771/fj.v1i1.34
Riskesdas. (2018). West sumatra province report riskesdas 2018. In basic health research 2018.
Sharma, D., Saxena, R., Namdeo, P., & Singh, P. (2021). Evaluation Of Antidiarrheal Activity Of Ethanolic Root Extract Of Glycyrrhiza Glabra Linn. In Rats. European Journal Of Biomedical And Pharmaceutical Sciences, 08, 181–187. https://doi.org/10.17605/OSF.IO/UFHYR
Sukmawati, I., Sukandar, E., & Kurniati, N. (2018). Aktivitas Antidiare Ekstrak Etanol Daun Suji (Dracaena Angustifolia Roxb). PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 14, 173. https://doi.org/10.30595/pharmacy.v14i2.1948
Susanti, D., Marcellia, S., Saputri, G., & Nabila, A. (2023). Uji Toksisitas Ekstrak Etanol Kulit Buah Mahoni (Swietenia mahagoni) Pada Larva Artemia Salina DENGAN METODE BSLT (Brine Shrimp Lethality Test). Jurnal Ilmu Kedokteran Dan Kesehatan, 10, 1405–1411. https://doi.org/10.33024/jikk.v10i1.8892
Wahid, A., Wardani, A., & Astuti, R. (2018). Uji Efek Antidiare Ekstrak Etanol Daun Sawo (Manilkara zapota L.) Terhadap Mencit Jantan Dengan Metode Transit Intestinal. Jurnal Ulul Albab, 22(2), 61–63. https://doi.org/10.31764/jua.v22i2.587
Yuliyanti, M., Husada, V. M. S., Fahrudi, H. A. A., & Setyowati, W. A. E. (2019). Detergen Cair Ekstrak Biji Mahoni ( Swietenia mahagoni ) Quality and Detergency Optimization , Liquid Detergent Preparation. Jurnal Kimia Dan Pendidikan Kimia, 4(2), 65–76. https://doi.org/10.20961/jkpk.v4i2.32750
