Antioxidant Activity In Pineapple Kombucha Tea (Ananas comosus L. Merr.)
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Abstract:
Background of study: Pineapple (Ananas comosus L. Merr) is known for being rich in vitamin C, B1, B6, and folic acid, and it possesses strong antioxidant properties effective against free radicals, which can cause cellular damage and lead to degenerative diseases.
Aims and scope of paper: This study focuses on testing the antioxidant activity of made from pineapple kombucha extract
Methods: The research scope includes preparing pineapple samples and producing kombucha through a 14-day fermentation process. The antioxidant activity of the kombucha is then tested using the DPPH method, with the IC50 value serving as an indicator of antioxidant capacity
Result: A lower IC50 value corresponds to higher antioxidant activity, and it was found that pineapple kombucha exhibits stronger antioxidant activity (IC50 7.51 μg/ml) compared to regular kombucha (IC50 39.46 μg/ml). This enhancement is due to the increased phenolic content and vitamin C during fermentation. The process of making pineapple kombucha involves boiling 2 liters of mineral water, adding 6 tea bags (10 grams) and 500 grams of sugar, then pouring the mixture into a growth jar. After cooling, 2 pieces of SCOBY are added, and the mixture is fermented for 14 days. Previous studies have demonstrated that regular kombucha tea has strong antioxidant activity with an IC50 value of 39.46 μg/ml. However, pineapple kombucha shows even higher antioxidant activity, with an IC50 value of 7.51 μg/ml.
Conclusion: This suggests that adding pineapple to kombucha not only enhances its flavor but also significantly increases its health benefits by boosting its antioxidant activity.
Keywords: Ananas comosus, Antioxidant, Fermentation, Kombucha, Pineapple
Copyright (c) 2025 oki febrian, Mukhlis Sanuddin
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Afgani, C. A., Sari, R. N., Nairfana, I., & Setiawat, V. R. (2022). Sosialisasi dan pelatihan pembuatan kombucha dari buah nanas di desa batu bulan kecamatan moyo hulu kabupaten sumbawa. Jurnal Agro Dedikasi Masyarakat (JADM), 3(2), 37–41. Google Scholar
Bait, Y., Umar, D. P., Anjelin, K., Mokodompit, Abdullah, M., Modanggu, L. W., & Usman, N. (2022). Analisis mutu irisan buah nanas beku selama penyimpanan. Prosidig Seminar Nasional Mini Riset Indonesia, 1(1). Google Scholar
Chauliyah, A. I. N., & Murbawani, E. A. (2015). Analisis Kandungan Gizi Dan Aktivitas Antioksidan Es Krim Nanas Madu. Journal of Nutrition College, 4, 628–635. https://doi.org/https://doi.org/10.14710/jnc.v4i4.10172
Dewi, A. P. (2018). Penetapan Kadar Vitamin C Dengan Spektrofotometri Uv-Vis Pada Berbagai Variasi Buah Tomat. JOPS (Journal Of Pharmacy and Science), 2(1), 9–14. https://doi.org/https://doi.org/10.36341/jops.v2i1.1015
Firdaus, S., C, A. I., Isnaini, L., & Aminah, S. (2020). “ Review ” Teh Kombucha Sebagai Minuman Fungsional dengan Berbagai Bahan Dasar Teh. Prosiding Seminar Nasional Unimus, 3, 715–730. Google Scholar
Handayani, V., Ahmad, A. R., Sudir, M., Etlingera, P., & Sm, R. M. (2014). Uji Aktivitas Antioksidan Ekstrak Metanol Bunga dan Daun Patikala ( Etlingera elatior ( Jack ) R . M . Sm ) Menggunakan Abstrak. Pharm Sci, 1(4), 86–93. https://doi.org/https://doi.org/10.7454/psr.v1i2.3321
Indriyah, S. N., Ayu, D., & Permatasari, I. (2023). Penetapan Kadar Fenolik Serta Uji Aktivitas Antioksidan Ekstrak Dan Fraksi Batang Bajakah Kalalawit ( Uncaria Gambir Roxb ) Dengan Metode Frap. Jurnal Kesehatan Tradisional, 1(2), 147–158. https://doi.org/https://doi.org/10.47861/usd.v1i2
Kusuma, A. E., & Yolanda, A. (2022). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Afrika ( Vernonia Arborea Buch-Ham ) Dengan Metode Dpph Antioxidant Activity Test Of Ethanol Extract Of African Leaf ( Vernonia arborea Buch-Ham ) Using DPPH. Jurnal Farmasi Sains Dan Obat Tradisional, 1(1), 34–40. https://doi.org/https://doi.org/10.62018/sitawa.v1i1.9
Lestari, & Sa’diyah, L. (2020). Karakteristik Kimia dan Fisik Teh Hijau Kombucha pada Waktu Pemanasan yang Berbeda Comparison of Physical Characteristics of Kombucha Green Tea at Different Heating Times. Journal of Pharmacy and Science, 5(1), 15–21. https://doi.org/https://doi.org/10.53342/pharmasci.v5i1.158
Mukti, R. H. (2023). Efek Konsentrasi Starter Pada Pembuatan Minuman Kombucha Berbahan Dasar Teh Hijau Dan Buah Anggur Merah. Skripsi. https://doi.org/http://etd.repository.ugm.ac.id/ EFEK
Rusli, N., Saehu, M. S., & Fatmawati. (2023). Aktivitas Antioksidan Fraksi Etil Asetat Daun Meistera chinensis dengan Metode DPPH (1,1 –difenil-2-pikrilhidrazil). Jurnal Mandala Pharmacon Indonesia (JMPI), 9(1), 43–48. https://doi.org/10.35311/jmpi.v9i1.296
Selvaraj, S., & Gurumurthy, K. (2023). An overview of probiotic health booster-kombucha tea. Chinese Herbal Medicines, 15(1), 27–32. https://doi.org/10.1016/j.chmed.2022.06.010
Sibarani, S. I. M., Yudistira, A., & Deby, A. (2020). Uji Aktivitas Antioksidan Spons Stylissa Sp. Dengan Menggunakan Metode Dpph (1,1-Difenil-2-Pikrilhidrazil) Antioxidant. Pharmacon, 9(3), 419–424. https://doi.org/https://doi.org/10.35799/pha.9.2020.30027
Wang, B., Rutherfurd-markwick, K., Zhang, X., & Mutukumira, A. N. (2025). Kombucha : Production and Microbiological Research. Foods, 11(21), 1–18. https://doi.org/https://doi.org/10.3390/foods11213456
Yuningtyas, S., Masaenah, E., & Telaumbanua, M. (2021). VITAMIN C DARI KOMBUCHA DAUN SALAM ( Syzygium polyanthum ( Wight ) Walp .). Jurnal Farmamedika, 6(1), 10–14. https://doi.org/https://doi.org/10.47219/ath.v6i1.116
Author Biography
Mukhlis Sanuddin, Sekolah Tinggi Ilmu Kesehatan Haraapan Ibu Jambi
